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. 2016 Mar 17;98(4):627–642. doi: 10.1016/j.ajhg.2016.02.008

Figure 7.

Figure 7

TFIIEα Phosphorylation Is Impaired in Non-proliferating TTD/XP-D Primary Fibroblasts

Immunoblot analysis of lysates obtained by directly scraping four normal (C3PV, C1609RM, C377RM, and C16354BE), six TTD/XP-D (TTD8PV, TTD11PV, TTD12PV, TTD22PV, TTD23PV, and TTD24PV), and six XP/XP-D (XP16PV, XP29BE, XP17BE, XP34BE, XP35BE, and XP17PV) fibroblast strains in Laemmli buffer. The levels of the upper (gray) and lower (white) forms of TFIIEα and of TFIIEβ (black) were normalized to γ-tubulin and expressed as percentage of the corresponding values in C3PV cells. The reported values are the means of at least two independent experiments. Bars indicate the SE (∗∗∗p < 0.0005; ns, not statistically significant; Student’s t test).