Fig. S5.

Construction of recombinant myxoma virus strains. The M156 coding sequence was partially replaced by a DNA cassette containing a monomeric red fluorescent protein (mRFP) encoding gene. The genomic region surrounding the M156R locus is shown in A. The region duplicated in the inverted terminal repeat is indicated. (B) The recombination plasmid to delete M156R contains a part of the M154L gene, the intergenic region between M154L and M156R, part of the M156R sequence, a poxvirus synthetic early/late (sE/L) promoter followed by the mRFP gene, followed by a part of the M156R sequence, the intergenic region between M156R and M008.1L and a part of M008.1L. To generate revertant M156R- (MYXV-M029L^KO/M156R^KOM156R^rev) and M156R-L98P- (MYXV-M029L^KO/M156R^KOM156R-L98P) containing strains, the constructs shown in C and D, respectively, were used.