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. 2016 Mar 21;113(14):E2019–E2028. doi: 10.1073/pnas.1522679113

Fig. 3.

Fig. 3.

Bud-site–selection proteins conditionally regulate the fMAPK pathway depending on glucose availability. (A) Budding pattern of haploid cells in glucose-rich and glucose-limiting conditions (Glu, glucose). Proteins required for axial budding, distal-unipolar budding, and the core module are shown. (B) Quantitative PCR (qPCR) analysis of FLO11 expression (relative to ACT1 levels) in the indicated mutants in glucose-rich (YEPD) and glucose-limiting (YEP-Gal) conditions. Assays were performed from independent cultures. Average values are shown. Error bars show the SD between trials. *P < 0.01. (C) P∼Kss1p levels were examined as in Fig. 1A for the indicated strains. Cells were grown to midlog phase in YEPD medium. NS, not significant. (D) Ratio of the long-to-short axis in the indicated mutants. More than 50 cells were counted for each mutant. Cells were incubated in glucose-limiting media (YEP + 0.2% glucose). *P < 0.001.