Fig. 8.

Theoretical predictions and experimental values of acetate consumed and CO₂ produced. A stoichiometric model was constructed to assess the efficiency of acetate utilization during the fermentation process, taking into account acetate consumed and CO₂ produced for both nonlipid biomass production (growth) and lipid production, including the associated ATP and NADPH costs of these processes. The experimental CO₂ production and acetate consumption are within 10% of the expected theoretical values. Model detail is provided in SI Notes on the Model.