Fig. S2.

(A) In a typical sonocrystallization, a 1-mL sample is slowly cooled until ∼−6 °C, after which a short sonopulse is applied to initiate freezing. The sonocrystallization measurements demonstrate a high reproducibility over the entire temperature profile, as shown by three repeat measurements of rQAE at a concentration of 1 mg/mL in 20 mM Tris, pH 7.5. The temperature readout accuracy of the experimental setup is within 0.01 °C. (B) Measurements on the AFP samples have high reproducibility with a deviation in determining the nonequilibrium freezing temperature below <0.02 °C.