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Caspase-2, caspase-3, caspase-6, caspase-7, and caspase-8 sequence specificity obtained by MSP-MS. Each caspase was incubated with a 228-member degenerate peptide library with the MSP-MS method to evaluate the protease specificity in vitro (28). (A) Total number of peptides cleaved by each enzyme (presence of aspartate at C-termini) after 5 and 24 h incubation. (B–F) Sequence recognition motifs of caspase-2, caspase-3, caspase-6, caspase-7, and caspase-8, respectively (P value = 0.05).
