Fig. 6.

(A) The decay of the M intermediate (black lines) and the recovery of the unphotolyzed state (blue lines) in the purified GtACR1_C102A mutant, and the decay of laser flash-induced photocurrent generated by this mutant in a HEK293 cell. Solid lines are experimental data; dashed lines, two-exponential fit. (B) The absorption spectrum of the intermediates from which the unphotolyzed state recovers in the GtACR1_C102A mutant obtained by subtraction of the difference spectrum between spectra measured at 1 s and 100 s from the properly scaled absorption spectrum of dark-adapted pigment.