Fig. 3.

TRPV4 is functionally expressed in NPE, but not PE, cells. (A–C) Dissociated cells. (A) The selective agonist GSK101 (25 nM) evokes ${\left[{\text{Ca}}^{2\mathrm{+}}\right]}_{\text{i}}$ signals in NPE (black trace; n = 15), but not PE (gray trace; n = 14), cells. (B) NPE cells. GSK101-evoked responses were observed in WT (n = 62) but not Trpv4^−/− cells (n = 41) and were antagonized by the selective blocker HC-06 (1 μM). (C) PE cells were unresponsive to GSK101 (WT, n = 54; KO, n = 34). (D and E) Representative time course of the calcium response in dissociated NPE (Top) and PE cell (Bottom) stimulated with GSK101. Note the melanin pigment in the PE cell. The timing of each calcium response in D is shown on the intensity time course in E (blue, NPE; red, PE). ***P < 0.005.