Fig. 4.

TRPV4 activation in NPE cells requires activation of the canonical PLA2 pathway. Dissociated cells. (A) AA-evoked ${\left[{\text{Ca}}^{2\mathrm{+}}\right]}_{\text{i}}$ elevations in NPE cells (n = 30) were antagonized by HC-06. AA had little effect on ${\left[{\text{Ca}}^{2\mathrm{+}}\right]}_{\text{PE}}$ levels (n = 28). (B) NPE cells. The PLA2 antagonist pBPB inhibits HTS-evoked ${\left[{\text{Ca}}^{2\mathrm{+}}\right]}_{\text{i}}$ signals (n = 25). (C) NPE cells. 11′,12′-EET induces ${\left[{\text{Ca}}^{2\mathrm{+}}\right]}_{\text{i}}$ elevations that are antagonized by HC-06 (n = 32).