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(A) The CB expresses mRNAs coding for multiple vanilloid TRP isoforms. Analysis of Trpv transcripts shows prominent signals for Trpv2 and Trpv4 primers and weaker signals for Trpv1, TRpv3, and Trpv6 primers (n = 3). (B) Semiquantitative real-time PCR analysis of mRNA levels in WT and Trpv4−/− CB tissue shows significant decreases in the relative abundance of Trpv1 and Trpv3 transcripts but no changes in the expression of housekeeping Gapdh and α-tubulin (Tuba1a) genes (n = 5).
