Table 4. Heart Rate Analysis of Mice.

Heart Rate (BPM)
Group	Tail Cuff	Echocardiography	Electrocardiogram
Control	742 ± 16	476 ± 9*	698 ± 22
T4	702 ± 13	575 ± 9	707 ± 22
DMSO + T4	706 ± 15	556 ± 14	669 ± 19
Sorafenib + T4	699 ± 13	600 ± 9	711 ± 15
TadalafilIP + T4	712 ± 14	551 ± 10	717 ± 18
TadalafilOr + T4	707 ± 17	611 ± 14	706 ± 7
CMC + T4	685 ± 12	592 ± 9	728 ± 16
MacitentanLD + T4	669 ± 20	568 ± 8	705 ± 26
MacitentanHD + T4	661 ± 14	585 ± 8	697 ± 11

BPM: beat per minute. Tail Cuff: Control; n = 10, Thyroxin (T4); n = 21, Dimethyl sulfoxide (DMSO); N = 6, Sorafenib; n = 13, Tadalafil_IP (intraperitoneal, 1 mg/kg); n = 7, Tadalafil_Or (oral, 4 mg/kg); n = 8, carboxymethylcellulose (CMC); n = 10, Macitentan_LD (Low dose: 30 mg/kg); n = 10, Macitentan_HD (High dose: 100 mg/kg); n = 8. Echocardiography: Control; n = 22, T4; n = 34, DMSO; N = 14, Sorafenib; n = 13, Tadalafil_IP; n = 22, Tadalafil_Or; n = 8, CMC; n = 10, Macitentan_LD; n = 10, Macitentan_HD; n = 8. Electrocardiogram Analysis: Control; n = 7, T4; n = 12, DMSO; N = 6, Sorafenib; n = 9, Tadalafil_IP; n = 7, Tadalafil_Or; n = 8, CMC; n = 10, Macitentan_LD; n = 9, Macitentan_HD; n = 7.

*: indicates a significant change as revealed by one-way ANOVA followed by Dunnett Multiple Comparisons post-hoc test, comparing all groups to T4.