Table 2. Performance statistics of the T. cruzi assay.

Robust Z-factor	0.8 +/- 0.1 (n = 344)
Coefficient of Variation [%]	9.8 +/- 2.8 (n = 344)
Signal to Background	31 +/- 13 (n = 344)
Percent Infected Cells	27% (n≈1x106)
Amastigotes / vero cell	6.8 +/- 3 (n = 344)
Throughput	40 x 384 well per batch

Values are averages +/- standard deviation, n is number of plates for Z-factor, %Coefficient of Variation, Signal:Background (S:B) and Amastigotes / vero cell (calculated from 32 control wells on each plate, includes both infected and non-infected cells) and number of Vero cells for Percent Infected Cells. Coefficient of Variance is for infected vehicle control wells. For S:B, signal is average number of amastigotes / Vero cell in vehicle control wells, background is average number of amastigotes / Vero cell in 100% effect (50μM Nifurtimox) control. Percent Infected cells and Amastigotes / Vero cell were determined at 72h.