Table 1.
Surface and nuclear marker expression in cultured ADSCs and MDCs
| Marker | Source | Class | Made in | 10 dilution | ADSCs | MDCs |
|---|---|---|---|---|---|---|
| Surface markers | ||||||
| CD73 | BD Biosciences | IgG1 | Mouse | 1:10 (FACS); 1:200 (IF) | +ve | NT |
| CD90 | BD Biosciences | IgG1 | Mouse | 1:10 (FACS); 1:200 (IF) | +ve | NT |
| CD34 | Novus Biologicals | IgG1 | Mouse | 1:10 (FACS); 1:200 (IF) | −ve | NT |
| Sca I | Abcam | IgG2a | Mouse | 1:10 (FACS); 1:200 (IF) | −ve | NT |
| Nuclear markers | ||||||
| Pax7 | DHSB | IgG1 | Mouse | 1:10 (FACS); 1:50 (IF) | −ve | +ve |
| MyoD | Dako Denmark A/S | IgG1 | Mouse | 1:10 (FACS); 1:50 (IF) | −ve | +ve |
| Myogenin (F5D) | DHSB | IgG1 | Mouse | 1:10 (FACS); 1:50 (IF) | −ve | +ve |
Notes: Source details: BD Biosciences (San Jose, CA, USA); Novus Biologicals (Littleton, CO, USA); Abcam (Cambridge, UK); DHSB (Iowa City, IA, USA); Dako Denmark A/S (Glostrup, Denmark).
Abbreviations: CD, cluster of differentiation; Sca I, stem cell antigen I; IgG, immunoglobulin G; 10, primary antibody; ADSCs, adipose-derived stem cells; MDCs, muscle-derived progenitor cells; FACS, fluorescence-activated cell sorting; IF, immunofluorescence; NT, not tested; +ve, positive; −ve, negative.