Fig 5. gB-gH/gL complexes form early after synthesis and are stable.
ARPE-19 cells were transduced with Ad vectors expressing either gB or gH/gL alone or with a combination of both gB and gH/gL. After 24 hrs, the cells were radiolabeled for 15 min. with 35S-methionine/cysteine then 1% NP-40 cell extracts made immediately (P) or the radiolabeled cells incubated in chase media with excess non-radioactive methionine and cysteine, i.e. the label chased (C) for 60, 120, or 240 min. After these chase periods, cell extracts were made immediately and proteins IP’d with anti-gH MAb 14-4b (IP: gH) or anti-gB rabbit serum (IP:gB) and analyzed by SDS-PAGE under reducing conditions. Molecular mass (MW) markers are indicated on the right.