Figure 3.

Mutant Allele Frequency in Capillary-Enriched Lobules Is Higher than That in the Surrounding Stromal Tissue within Congenital Hemangioma

(A) Pre-laser capture image of NICH showing multiple dermal vascular lobules from participant 4 (staining dark blue in hematoxylin and eosin-stained section, ×20 magnification).

(B) Post-laser capture image of the section depicted in (A) showing absence of virtually all vascular lobules (hematoxylin and eosin-stained section, ×20 magnification).

(C) Pre-laser capture of skeletal muscle, fibrous tissue, and fat uninvolved tissue by the NICH shown in (A) and (B) (hematoxylin and eosin-stained section, ×20 magnification) that was resected en bloc.

(D) Graph depicting the GNAQ c.626A>T allele frequencies in DNA extracted from the capillary enriched lobules (A), the residual tissue (B), and the adjacent muscle tissue (C) measured in triplicate by ddPCR (dots). Solid lines indicate the average of the three measurements.