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. 2015 Sep 23;12(12):1314–1322. doi: 10.1080/15476286.2015.1094600

Figure 6.

Figure 6.

Over-expression of miR-152 alleviates bone destruction in MM in vivo. (A) Stably expressing miR-152 or empty vector infected MM.1S cells were injected into bone marrow of mice for the development of MM. Levels of miR-152 (left panel) and DKK1 mRNA and protein (right panel) are obtained from the mice bone marrow, when the disease is confirmed. Statistical significances at **p < 0.001 vs. empty vector from 3 independent experiments. (B) H&E staining showing equal and successful injection of 0.5×106 VecMM.1S and miR-152MM.1S cells into mice bone marrow. (C) Representative immunohistochemical staining of DKK1 (upper panel) and CD138+ (lower panel) in the VecMM.1S and miR-152MM.1S cells obtained from the mice bone marrow. (D) Representative fluorescent micrographs of dual calcein labeling of femur bone from 2 mice injected with 0.5×106 VecMM.1S and miR-152MM.1S cells. The distance between the 2 calcein labeling layers reflects the bone mineralization rate, and the analysis of the intensity ratio between the VecMM.1S group and the miR-152MM.1S groups were shown at the bottom. Bars represent the mean ± SEM of n = 10 mice per group (*P < 0.05).