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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Crit Care Med. 2016 May;44(5):e241–e252. doi: 10.1097/CCM.0000000000001397

Figure 4. Blood chemistry changes for renal function and Megalin staining of renal proximal tubules.

Figure 4

Blood chemistry tests were performed at day 0 (pre burn), day 10 pb (before LPS), and day 11 pb (24 hours after LPS) (A–F: N=3 rats total, N=2–3 experiments for each sham burn group; N=6 rats total, N=3–4 experiments for each burn group). A, B. The renal function marker, blood urea nitrogen (BUN), increased significantly after LPS injection, and restored by RvD2 pre treatment (A: Sham Burn/LPS vs. Sham Burn/LPS/RvD2; *p≤0.05). In burned rats, BUN increased more significantly after the second septic insult (Burn/LPS vs. Burn/LPS/RvD2; ***p≤0.001). C, D. Serum creatinine values did not change in any of the groups. E, F. In sham burn groups, serum albumin values were preserved during treatment period, and decreased after LPS, in both treated and untreated groups. The decrease in serum albumin after LPS was ameliorated by RvD2 treatment (E: Sham Burn/LPS vs. Sham Burn/LPS/RvD2; *p≤0.05). On the other hand, levels of serum albumin decreased after burn and even further after LPS, in both treated and untreated groups. The decrease in serum albumin after burn and burn and LPS was also ameliorated by RvD2 treatment (F: Burn vs. Burn/RvD2; †p≤0.05, Burn/LPS vs. Burn/LPS/RvD2; *p≤0.05). G, H. Representative images of renal proximal tubules at day 11 pb in burn only group (G) and burn + RvD2 treatment group (H). Green fluorescence shows megalin, and the blue fluorescence shows the nuclei. Megalin is localized in the apical brush border region of the proximal tubule. Staining intensity after burn injury is higher in the RvD2 treated group. Scale bars, 50 μm. I. Quantitative analysis of fluorescence intensity of megalin staining. Megalin intensity decreased after burn and LPS groups, and was restored in the burn and RvD2 treatment group, but not in the burn and LPS and RvD2 group (Sham Burn and Burn groups N=3 rats total; all other groups N=4 rats total).