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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Pancreas. 2016 May-Jun;45(5):659–670. doi: 10.1097/MPA.0000000000000522

Figure 1. Effect of TGF-β on PTHrP mRNA and protein levels in acinar cells and PSCs.

Figure 1

(A,D) Cells were treated with TGF-β (3 ng/ml) for the indicated time intervals. PTHrP mRNA levels were measured by reverse transcription/real-time PCR. Values are expressed relative to the vehicle control value, set arbitrarily at 1.0. (B,E) Western blot analysis for PTHrP in cells treated with TGF-β (1 or 3 ng/ml) for 18 h. − = vehicle control. The figure is representative of data obtained from 3 independent experiments. (C,F) Densitometric analysis of Western blots. 10 = primary cells. Values are expressed relative to the 0 time point (A, D) or the −TGF-β control value (C, F), set arbitrarily at 1.0. Each point or bar is the mean ± SEM of three independent experiments. ***, P < 0.001 vs. 0 time-point (A,D); **, P < 0.01 vs. control (C,F).