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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Pancreas. 2016 May-Jun;45(5):659–670. doi: 10.1097/MPA.0000000000000522

Figure 6. Effect of inhibiting PTHrP signaling on the TGF-β-mediated increase in gremlin levels in acinar cells.

Figure 6

(A) Cells were pre-treated with 10−5 M PTHrP (7-34) for 1 h, then treated in the presence of PTHrP (7-34) with TGF-β (3 ng/ml) for 2 h. 10 = primary cells. (B) Gremlin levels in acinar cells from PTHrPΔacinar mice and PTHrP+/+ mice treated with TGF-β for the indicated time intervals. (A,B) Gremlin mRNA levels were measured by reverse transcription/real-time PCR. Values are expressed relative to the control value (A) or the PTHrP+/+ 0 time-point (B), set arbitrarily at 1.0. Each bar or point is the mean ± SEM of three independent experiments. ***, P < 0.001 vs. control (A) or 0 time-point (B); #, P < 0.01 vs. TGF-β alone (A) or PTHrP+/+ value (B).