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Primary (10) PSCs and irPSCc3 cells were treated with BMP-2 (50 ng/ml) for the indicated time intervals. PTHrP mRNA levels were measured by reverse transcription/real-time PCR. Values are expressed relative to the vehicle control value, set arbitrarily at 1.0. Each point is the mean ± SEM of three independent experiments. ***, P < 0.001 vs. 0 time-point.
