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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: J Comp Neurol. 2016 Jan 4;524(9):1892–1919. doi: 10.1002/cne.23946

Figure 6. VGLUT1-immunoreactive contacts on Renshaw cells in the ventral horn of wild-type, Egr3(−/−) and mlcNT3(+/−) P20 mice.

Figure 6

A1, B1, C1, Low magnification confocal images of VGLUT1-IR boutons (green, FITC) and CB-IR RCs (magenta, Cy3) in the ventral horn at P20 in wild-type (A1), Egr3(−/−) (B1) and mlcNT3(+/−) (C1) animals. The yellow dotted line indicates the border between the ventral horn and the white matter. A2, B2, C2, Superimposed VGLUT1-IR (green) and CB-IR neurons (magenta, Cy3). Boxes indicate areas shown at high magnification in A3, B3, and C3. A3-5, B3-5, C3-5, VGLUT1-IR boutons on P20 CB-IR RCs surface rendered in Imaris software and shown in three different rotations. Arrowheads indicate the same VGLUT1-IR contacts on RC dendrites at three different rotations. Compared to wild-types there are fewer VGLUT1-IR contacts in Egr3(−/−) RCs and more in mlcNT3(+/−). Scale bars: 100 μm in A1 (A1-A2, B1-B2 and C1-C2 are all at the same magnification); 20 μm in A3 (A3, B3 and C3 are at the same magnification).