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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: J Comp Neurol. 2016 Jan 4;524(9):1892–1919. doi: 10.1002/cne.23946

Figure 8. VAChT-immunoreactive contacts on Renshaw cells in the ventral horn of wild-type, Er81(−/−) Egr3(−/−), and mlcNT3(+/−) P20 mice.

Figure 8

A1, B1, C1, D1, Low magnification confocal images of VAChT-IR boutons (green, FITC) and CB-IR RCs (magenta, Cy3) in the ventral horn of a P20 wild-type (A1), Er81(−/−) (B1), Egr3(−/−) (C1), and mlcNT3(+/−) (D1) animal. VAChT-IR staining is increased in LIX and in the RC area of Egr3(−/−) and Er81(−/−) mice compared to wild-types. The yellow dotted line indicates the border between the ventral horn and white matter. A2, B2, C2, D2, Superimposed VAChT-IR (green) and CB-IR (magenta, Cy3). Boxes indicate areas of high magnification shown in A3, B3, C3, and D3. A3-5, B3-5, C3-5, D3-5, VAChT-IR contacts on P20 CB-IR RCs surface rendered in Imaris software and shown in three different rotations. Arrowheads indicate VAChT-IR contacts on RC dendrites, shown in different rotations. There appears to be more VAChT-IR contacts on RCs in Er81(−/−) (B3) and Egr3(−/−) (C3) mice compared to wild-type control (A1) animals. In contrast, RCs in mlcNT3(+/−) (D3) mice have fewer VAChT-IR contacts than wild-types. Scale bars: Scale bars: 100 μm in A1 (A1-A2, B1-B2 C1-C2 and D1-D2 are all at the same magnification); 20 μm in A3 (A3, B3 C3 and D3 are at the same magnification).