Table 1. Antibodies used in this study.
| Antigen | Immunogen | Host/Type | Manufacturer | Dilution |
|---|---|---|---|---|
| Calbindin | Rat recombinant Calbindin D28k |
Rabbit polyclonal |
Swant (Bellizona, Switzerland), cat. no. CB-38a, lot no. 9.03, |
1:2000-1:5000 |
| NeuN | Purified cell nuclei from mouse brain |
Mouse monoclonal |
Chemicon (Temecula, CA), cat. no. MAB377B, lot no.25040759, |
1:500-1:1,000 |
| Parvalbumin | Purified from frog muscle |
Mouse monoclonal |
Chemicon (Temecula, CA), cat. no. MAB1572, lot no. 070105877, |
1:1000 |
| VAChT | Synthetic peptide from rat VAChT (20 aa: 511-530) |
Goat polyclonal |
Chemicon (Temecula, CA), cat. no. AB1578, lot no.0602021588, |
1:1000-1:2000 |
| VGLUT1 | Synthetic peptide from rat VGLUT1 (18 aa: 542-560) |
Guinea pig polyclonal |
Chemicon (Temecula, CA), cat. no. AB5905, lot no. 0507005922 |
1:1000-1:2000 |
| VGLUT2 | Purified recombinant protein of rat VGLUT2 (72 aa: 510-582) |
Guinea pig polyclonal |
Synaptic Systems (Goettingen, Germany), cat. no. 135 404 lot no. 135404/3 |
1:1000 |
Abbreviations: NeuN, Neuronal Nuclear Protein; VAChT, Vesicular Acetylcholine Transporter; VGLUT1, Vesicular Glutamate Transporter 1; VGLUT2, Vesicular Glutamate Transporter 2;