Extended Data Figure 10. RIP3 and Mincle signaling are necessary for macrophage-induced suppression of T cell immunity in PDA.

(a–c) Cohorts of WT, RIP3^−/−, and Mincle^−/− animals were challenged with orthotopic PDA and serially treated with (a) a neutralizing αCD90 mAb, (b) a neutralizing αF4/80 mAb, or isotype control. Mice were sacrificed at 21 days and pancreatic tumors weighed. Controls were shared for both experiments and are shown twice (n=4 for Mincle^−/− αCD90 and αF4/80-treated groups and n=3 for other groups). (c) CD4⁺ and CD8⁺ T cell activation was determined by expression of CD44 in WT, RIP3^−/−, and Mincle^−/− cohorts treated with αF4/80 or isotype control. Graphs show mean ± s.e.m. *p<0.05, **p<0.01, ***p<0.001, ****p<0.001 (unpaired, t-test). In vivo cellular depletion experiments were repeated on 2 separate occasions with similar results. (d) Schematic depicting immune-suppressive implications of RIP1/RIP3-driven CXCL1 expression and Mincle activation.