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. Author manuscript; available in PMC: 2016 Oct 4.
Published in final edited form as: Nature. 2016 Apr 4;532(7598):250–254. doi: 10.1038/nature17392

Extended Data 4. β-catenin loss in the aged microenvironment.

Extended Data 4

β-catenin expression in normal human skin from young and aged donors, with a focus on the fibroblast population (zoom) (a). β-catenin nuclear translocation in melanoma cells treated with conditioned media from aged as compared to young fibroblasts as measured by Western analysis (b) and a TOPFLASH assay. Two-tailed unpaired t test was performed to indicate statistical significance between treatment with young and aged media (p=0.023). Data are represented as mean ± s.d. (c). Immunofluorescent analysis of β-catenin in melanoma cells treated with media from young fibroblasts in which the β-catenin is knocked down (d).