Skip to main content
. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Biomaterials. 2016 Mar 23;92:57–70. doi: 10.1016/j.biomaterials.2016.03.034

Figure 8.

Figure 8

Effect of nanofibers (horizontally, randomly, and perpendicularly aligned with respect to the gel surface) on zone-specific chondrogenic expression of mRNA and biochemical markers for differentiating hMSCs. mRNA markers were SZP (a), Sox-9 (b), AGC (c), Col II (d), Col X (e), and ALP (f). Biochemical markers were total collagen (g), GAG (h), and ALP (i). hMSCs were encapsulated in L1 (red), L2 (blue), and L3 (green) gels simulating the superficial, middle, and calcified zones of articular cartilage, respectively, and cultured in chondrogenic medium with (solid lines) or without (dashed lines) nanofibers. L3 gel did not have a “no fiber” group in (a–i) because fibers had to be added to Calcified gel to reach the matrix modulus of 320 MPa. The pink, brown, and black diamonds show mRNA marker expression levels of hMSCs (day zero), chondrocytes, and pellet-cultured hMSCs (day 14), respectively, as controls. An asterisk represents a statistically significant difference (P<0.05) between the test group and all other groups for the given time point. The colored “+” sign represents a statistically significant (P<0.05) positive effect of fibers on the zone corresponding to that color (red for superficial, blue for middle, and green for calcified) for all time points. The colored “−” sign represents a statistically significant (P<0.05) negative effect of fibers on the specified zone for all time points. Error bars correspond to means ± 1 SD for n=3.