Table 1.
Gel composition, nanofiber orientation and loading, in vitro gel degradation (mass loss), gel compressive modulus, growth factor concentrations, and hMSC cell density for hydrogel constructs simulating the superficial, middle, and calcified zones of articular cartilage.
| Simulated cartilage zone | Gel composition | Fiber orientation & loading (wt. %) | In-vitro Mass loss after 21 days | Matrix modulus | Growth factors | Cell density (Cells/ml) |
|---|---|---|---|---|---|---|
| Superficial | SPELA10 (15%) | Horizontal 4% |
47% | 80 KPa | TGF-β1 (3 ng/ml) BMP-7 (100 ng/ml) |
60×106 |
| Middle | SPELA7.5 (50%) | Random 4% |
38% | 2.1 MPa | TGF-β1 (30 ng/ml) IGF-7 (100 ng/ml) |
20×106 |
| Calcified | SPELA5 (35%) | Vertical 60% |
28% | 320 MPa | TGF-β1 (30 ng/ml) HA (3%) |
15×106 |