A. Simulations were performed using the variables N0, p, and R values of {1, 0.2, R}, where R varies from 0.001 to 0.1. The last fifteen points of cumulative EPSCs were fit with a line and backextrapolated to x=0 to measure RRPtrain. B. Plots of the simulated EPSC amplitude versus cumulative EPSC are shown. Linear fits to the first four points of each curve are extended to y=0, indicating the value of RRPEQ. C. The measured pool size as a function of replenishment rate for two methods of RRP estimation: RRPtrain (red) and RRPEQ (blue). The actual RRP size is equal to one (dotted black line).