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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Crit Care Med. 2016 May;44(5):e289–e299. doi: 10.1097/CCM.0000000000001566

Fig. 6. Sodium transporter gene expression in the kidney in sham and septic mice.

Fig. 6

A. Impact of cfB deficiency on the sodium transporter gene expression. WT and cfB−/− mice were subjected to sham or CLP procedures. Mice were euthanized and kidneys harvested 12 hours later. NKCC2, Na+/K+ ATPase-α1, ROMK, ENaC-α, and NHE3 gene expression were determined by qRT-PCR. *P<0.05, **P<0.01, ***P<0.001. N=4 in Sham group, N=6–7 in CLP group. B. Effect of anti-cfB antibody on sodium transport gene expression. One mg of mouse control IgG or anti-cfB antibody mAb1379 was administered i.p.1 hour before sham or CLP surgery. The kidneys were harvested 12 hours later and RNA extracted. NKCC2 and Na+/K+ ATPase-α1 gene expression were determined by qRT-PCR. N=7 in sham or CLP group. **P<0.01. NKCC2 = Na+-K+-2Cl− cotransporter, ROMK = renal outer medullary potassium channel, NHE3 = Na+/H+-exchanger, ENaC-α = epithelial sodium channel.