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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: J Comp Neurol. 2015 Dec 29;524(9):1859–1875. doi: 10.1002/cne.23926

Figure 1.

Figure 1

Western blot of BRP antibody using Manduca brain tissue and pre-adsorption controls for the 5-HT, GABA, MIP and TKK antibodies. A: Western blots of Manduca brain tissue with the BRP antibody resulted in a single band at the predicted height for the Manduca homologue of the ELKS/CAST protein protein (~234kDa). The BRP antibody was used to mark glomerular outlines. B: Pre-adsorption of the goat anti-5-HT antiserum by incubating with 1mg/mL BSA and 10:1 5-HT-BSA conjugate:goat anti-5-HT antibody for 24hrs at 4°C abolished all staining of Manduca AL tissue. C: Non-pre-adsorbed controls in which goat anti-5-HT antibody was incubated in parallel under identical conditions with the exception of the 5-HT-BSA conjugate resulted in strong immunolabeling. D: Pre-adsorption of the rabbit anti-GABA antiserum by incubating with 1mg/mL BSA and 10:1 GABA:rabbit anti-GABA antibody for 24hrs at 4°C abolished all staining of Manduca AL tissue. E: Non-pre-adsorbed controls in which rabbit anti-GABA antibody was incubated in parallel under identical conditions with the exception of GABA resulted in strong immunolabeling. F: Pre-adsorption of the rabbit anti-MIP antiserum by incubating with 1mg/mL BSA and 10:1 synthetic MIPVI (AWSALHGAWA): rabbit anti-MIP antibody for 24hrs at 4°C abolished all staining of Manduca AL tissue. G: Non-pre-adsorbed controls in which rabbit anti-MIP antibody was incubated in parallel under identical conditions with the exception of the synthetic MIPVI resulted in strong immunolabeling. H: Pre-adsorption of the rabbit anti-TKK antiserum by incubating with 1mg/mL BSA and 10:1 synthetic TKKI (RAPMGFMGVR): rabbit anti-TKK antibody for 24hrs at 4°C abolished all staining of Manduca AL tissue. I: Non-pre-adsorbed controls in which rabbit anti-TKK antibody was incubated in parallel under identical conditions with the exception of the synthetic TKKI resulted in strong immunolabeling. Scale bar = 100 um.