Table 1.
Bioactive LM profile of human milk (4-8 weeks post-partum)*
| Healthy human milk, 4-8 wks post-partum |
||||
|---|---|---|---|---|
| DHA Bioactive | Lipid mediator levels | |||
| SPM Metabolome | Q1 | Q3 | (pg/mL) | pM |
| RvD1 | 375 | 215 | 147 ± 47.2 | 391 |
| AT-RvD1 | 375 | 215 | 67.4 ± 11.7 | 180 |
| RvD2 | 375 | 215 | 82.4 ± 28.0 | 219 |
| RvD3 | 375 | 147 | 7.2 ± 2.7 | 19.1 |
| AT-RvD3 | 375 | 147 | 15.0 ± 2.9 | 39.9 |
| RvD4 | 375 | 101 | 27.4 ± 7.5 | 72.9 |
| RvD5 | 359 | 199 | 19.9 ± 8.9 | 52.9 |
| RvD6 | 359 | 101 | 6.7 ± 2.4 | 17.8 |
| PD1 | 359 | 153 | 4.3 ± 2.3 | 11.9 |
| AT-PD1 | 359 | 153 | 3.8 ± 0.9 | 10.6 |
| MaR1 | 359 | 221 | 20.8 ± 6.3 | 57.8 |
| AA Bioactive LM | ||||
| Metabolome | ||||
| LXA4 | 351 | 115 | 25.7 ± 8.6 | 72.9 |
| AT-LXA4 | 351 | 115 | 370.0 ± 176.6 | 1260 |
| LXB4 | 351 | 115 | 267.1 ± 93.9 | 759 |
| AT-LXB4 | 351 | 115 | - | - |
| LTB4 | 335 | 195 | - | - |
| PGE2 | 351 | 189 | 409.7 ± 146.6 | 1160 |
| PGD2 | 351 | 189 | 568.3 ± 188.9 | 1610 |
| PGF2α | 353 | 193 | 111.1 ± 36.2 | 314 |
| TxB2 | 369 | 169 | 111.8 ± 44.4 | 302 |
| EPA Bioactive | ||||
| SPM Metabolome | ||||
| RvE1 | 349 | 195 | 8.8 ± 3.6 | 25.1 |
| RvE2 | 333 | 253 | 321.2 ± 129.2 | 962 |
| RvE3 | 333 | 201 | 444.9 ± 179.8 | 1330 |
*
Quantification of bioactive lipid mediators (LM) in human milk (4-8 weeks post-partum) assessed by LC-MS-MS based LM metabololipidomics. Results are expressed as pg/mL human milk. Q1: M-H (parent ion) and Q3 (daughter ion): diagnostic ion in the MS-MS. Detection limit was ~ 0.1 pg; - denotes below limits. Results are mean ± s.e.m. from 4 donors. Complete LC-MS-MS, retention times and MS-MS spectra for each eicosanoid and SPM listed here are presented in Supplemental Figure 1.