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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Mucosal Immunol. 2015 Oct 14;9(3):744–756. doi: 10.1038/mi.2015.98

Figure 7. Differentiated LRRC31-overexpressing EPC2 cell transcriptome.

Figure 7

a, Heat diagram representing RNA sequencing analysis of differentiated empty vector (control) and LRRC31-overexpressing EPC2s. Differentially expressed genes were identified by filtering on FPKM ≥ 1, moderated t-test with Benjamini-Hochberg False Discovery Rate (P < 0.05), and fold change ≥ 1.5. KLK family members are bolded. b, Normalized KLK1, KLK5, KLK7, KLK11, and KLK13 mRNA expression in differentiated control and LRRC31-overexpressing (LR) EPC2s determined by qPCR analysis. A representative experiment is shown (n = 3). c, Total protein normalized KLK10, KLK5, KLK7, KLK11, and KLK13 protein expression in differentiated control and LRRC31-overexpressing EPC2 supernatants determined by protease array analysis. A representative experiment is shown (n = 3). d, KLK serine protease activity in differentiated control and LRRC31-overexpressing EPC2 supernatants (AFU, arbitrary fluorescence units normalized to total protein). A representative experiment is shown (n = 3). For b-d, data are represented as the mean ± SEM; NS, not significant; *P < 0.05, **P < 0.01, ****P < 0.0001.