Skip to main content
. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Mucosal Immunol. 2015 Oct 14;9(3):744–756. doi: 10.1038/mi.2015.98

Figure 8. KLK expression following LRRC31 gene-silencing in differentiated EPC2 cells.

Figure 8

EPC2 esophageal epithelial cells transduced with either non-silencing control (NSC) or LRRC31 short hairpin RNA (LRRC31 shRNA) lentiviral expression constructs were cultured as described in Figure 5b. a, Normalized LRRC31 and CCL26 mRNA expression in differentiated NSC and LRRC31 shRNA EPC2s at day 14 determined by qPCR analysis. Dashed line represents limit of detection. b, Fold change in LRRC31 mRNA expression following IL-13–treatment in differentiated NSC and LRRC31 shRNA EPC2s at day 14. c, H&E-stained sections from differentiated NSC and LRRC31 shRNA EPC2s at day 14. d, Normalized KLK1, KLK5, KLK7, KLK11, and KLK13 mRNA expression in IL-13–treated differentiated NSC and LRRC31 shRNA EPC2 cells at day 14 determined by qPCR. e, TER measured across differentiated NSC and LRRC31 shRNA EPC2s at day 14. RT, resistance. For a-e, a representative experiment is shown (n = 3). For a, b, d, and e, data are represented as the mean ± SEM; NS, not significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.