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. 2016 Apr 12;110(7):1485–1498. doi: 10.1016/j.bpj.2016.02.026

Figure 7.

Figure 7

Analysis of pressure-induced changes in the EPR spectra of CYP3A4(C409)R1 by TFA-PCA. (a) Approximation of the two pressure-induced transitions observed in fluorescence spectroscopy experiments (dashed lines), with linear combinations of the sets of loading factors for the first two PCs obtained in a pressure-perturbation experiment with CYP3A4(C409)R1. The main (reversible) transition is shown in circles, and the transition corresponding to apparent inactivation is shown in triangles. The solid lines represent the fitting of the data sets with Eq. 4. (b and c) Sets of EPR spectra obtained from the original data by sorting out the changes corresponding to reversible (b) and irreversible (c) components. The spectra shown in (b) represent pressures of 1, 1000, 2000, and 3000 bar, and those in (c) correspond to 1, 2000, 2400, and 3000 bar. The insets show the respective spectra of the first PCs of the pressure-induced changes.