Table 1.
FRET Efficiencies and Fractional Populations Obtained by Gaussian Decomposition of drkN SH3 smFRET Histograms in Denaturant
| [GdmCl] (M) | Cluster 1 | Cluster 2′ | Cluster 2 |
|---|---|---|---|
| 0 | 52.6 ± 21.5 (0.59) | – | 98.5 ± 2.5 (0.41) |
| 1 | 50.5 ± 28.6 (0.61) | 94.1 ± 5.2 (0.22) | 98.9 ± 2.3 (0.17) |
| 2 | 40.4 ± 25.3 (0.58) | 89.1 ± 7.9 (0.22) | 97.5 ± 3.6 (0.21) |
| 4 | 31.7 ± 18.8 (0.53) | 90.5 ± 6.7 (0.21) | 97.8 ± 2.9 (0.25) |
| 6 | 17.8 ± 16.2 (0.62) | 89.6 ± 6.8 (0.19) | 96.5 ± 4.0 (0.19) |
| 8 | 20.8 ± 17.9 (0.69) | 88.4 ± 7.3 (0.15) | 96.6 ± 3.8 (0.16) |
| [Urea] (M) | |||
| 1 | 29.7 ± 19.5 (0.72) | 92.8 ± 6.0 (0.12) | 99.1 ± 2.4 (0.16) |
| 3 | 19.8 ± 17.7 (0.80) | 91.7 ± 7.1 (0.10) | 99.1 ± 3.0 (0.10) |
| 7 | 12.3 ± 11.9 (0.69) | 91.7 ± 6.6 (0.18) | 98.2 ± 3.1 (0.13) |
Data are formatted as a percentage of the average FRET efficiency ± the half-width at half-maximum (HWHM). For each FRET peak (cluster), the fractional population is given in parentheses. See Fig. 2 for the histograms used to obtain the data.