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. 2016 Apr 12;110(7):1563–1573. doi: 10.1016/j.bpj.2015.12.043

Figure 1.

Figure 1

Detection of membrane order by average lifetime of domain-selective probes as a function of temperature in binary and ternary bilayers. 1 mol% of either tPA-SM (measuring order in the ordered domain) or O-tPA-PC (measuring order in the disordered domain) was included in the bilayers. The ternary bilayers (black) contained PSM/POPX/Chol (34.5: 34.5: 30 mol%) and the binary bilayers (white) contained POPX/Chol or PSM/Chol (53: 47 mol%). (A) shows data for POPC mixtures, (B) for POPE, and (C) for POPS mixtures; all panels included a comparison to the PSM/Chol mixture. Values are average ± SD of n = 3 (error bar may be smaller than symbol). For more details on the lifetimes, see Tables S1 and S2.