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Cholesterol-induced ordering of perdeuterated lipids in complex bilayers at 20°C (A) and 50°C (B). Ordering was measured as the first spectral moment (M1). MLVs were prepared to 50% hydration with the composition PSM/POPC/POPE/POPS 22:44:17:17 with the addition of 10–31 mol % cholesterol. To see this figure in color, go online.
