Dynamics of growth and differentiation within traps. (a–c) Time-lapse image of competence development using a PcomKgfp reporter in traps with (a) strong, (b) medium, and (c) weak coupling. Brightfield and fluorescence images were merged. Since the cells were not fixed, time elapsed between acquisitions of the brightfield and fluorescence images, and therefore the overlap is not always perfect. To see this figure in color, go online.