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. 2016 Apr 16;23:40. doi: 10.1186/s12929-016-0257-0

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© Banerjee et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Cell cycle histograms of the DNA content of MDA-MB-231 in response to andrographolide. Cells were incubated for 24 (b), 36 (c) and 48 (d) h time points in the absence (control, 48 h) (a) or presence of andrographolide (30 μM), prepared for FACS analysis, as described in Materials and Methods, and analyzed in FACSVerse™ (Becton Dickinson, USA) flow cytometer. The number of cells in each phase of the cell cycle was obtained by using FACSuite™ software. e The percentage of cells in each phase was shown as the mean ± S.D. from three independent experiments (*P < 0.05, **P < 0.01 and ***P < 0.001)