Fig. 6.

Western blot analysis of some important apoptosis associated proteins in MDA-MB-231 cells. Cells were treated with vehicle control (0.1 % DMSO) or with andrographolide (30 μM) for 48 h. Cells were then lysed and cell lysate were used for Western blot analysis as described in materials and methods. Data shown are representatives of three independent experiments. a Western blot analysis of different pro- and anti-apoptotic proteins. Expressions of Bax, Bcl-2, Bcl-xL and Apaf-1 in total cell lysate were detected after treatment. Band intensities were quantified by ImageJ and normalized to β-Actin which was used as loading control. Data were expressed as a band intensity relative to control and shown below [means ± S.D., n = 3; *P < 0.05, **P < 0.01, ***P < 0.001 compared with control]. b Effect of andrographolide on expression of cytochrome c in cytosolic and mitochondrial fraction in Western blot analysis. β-Actin and COX IV were detected as loading control respectively. Band intensities were quantified by ImageJ and normalized to respective loading controls. Data were expressed as a band intensity change compared to control and shown below [means ± S.D., n = 3; *P < 0.05, **P < 0.01, ***P < 0.001 compared with control]