Table 1.
List of identified GLYI genes in Soybean (Glycine max) along with their detailed information and localization
| Name | Gene | Protein | Chro. no | CDS coordinate (5’ to 3’) | CDS (bp) | Exons | PP length (aa) | MW (kDa) | pI | Localization |
|---|---|---|---|---|---|---|---|---|---|---|
| GmGLYI-1 | Glyma.01 g146300 | Glyma.01 g146300.1 | 1 | 48150827–48154366 | 1044 | 9 | 347 | 39.3 | 7.01 | Cha,c; Mtb; Cyb |
| Glyma.01 g146300.2 | 1038 | 9 | 345 | 39.1 | 7.01 | Cha,c; Mtb; Cyb | ||||
| Glyma.01 g146300.3 | 930 | 8 | 309 | 35.1 | 8.44 | Cha,b,c; Mtb; Cyb | ||||
| GmGLYI-2 | Glyma.01 g168400 | Glyma.01 g168400.1 | 1 | 50605679–50608020 | 579 | 3 | 192 | 22.0 | 5.04 | Cya; Ecb |
| GmGLYI-3 | Glyma.04 g083100 | Glyma.04 g083100.1 | 4 | 7006088–7009361 | 1041 | 9 | 346 | 38.5 | 5.83 | Cha,b,c |
| GmGLYI-4 | Glyma.05 g228500 | Glyma.05 g228500.1 | 5 | 40646576–40651812 | 1101 | 9 | 366 | 40.6 | 8.17 | Cha,b,c |
| Glyma.05 g228500.2 | 1089 | 9 | 362 | 40.2 | 6.28 | Cha,b,c | ||||
| GmGLYI-5 | Glyma.06 g084500 | Glyma.06 g084500.1 | 6 | 6498718–6501298 | 792 | 8 | 263 | 29.7 | 5.45 | Cyb |
| GmGLYI-6 | Glyma.07 g031700 | Glyma.07 g031700.1 | 7 | 2508024–2510223 | 519 | 2 | 172 | 19.6 | 5.10 | Nua; Cyb |
| Glyma.07 g031700.2 | 369 | 3 | 122 | 13.9 | 4.89 | Cya,b | ||||
| Glyma.07 g031700.3 | 381 | 2 | 126 | 14.5 | 5.03 | Cya,b | ||||
| GmGLYI-7 | Glyma.07 g261400 | Glyma.07 g261400.1 | 7 | 43645750–43649851 | 843 | 7 | 280 | 31.6 | 5.62 | Cya,b |
| Glyma.07 g261400.2 | 732 | 8 | 243 | 27.2 | 5.34 | Cya,b | ||||
| Glyma.07 g261400.3 | 795 | 7 | 264 | 29.8 | 5.24 | Cya,b | ||||
| Glyma.07 g261400.4 | 843 | 8 | 280 | 31.6 | 5.62 | Cya,b | ||||
| Glyma.07 g261400.5 | 843 | 8 | 280 | 31.6 | 5.62 | Cya,b | ||||
| Glyma.07 g261400.6 | 819 | 8 | 272 | 30.7 | 6.11 | Cya,b | ||||
| GmGLYI-8 | Glyma.08 g035400 | Glyma.08 g035400.1 | 8 | 2811954–2818455 | 1071 | 9 | 356 | 39.6 | 6.56 | Cha,b,c |
| GmGLYI-9 | Glyma.08 g211100 | Glyma.08 g211100.1 | 8 | 17046316–17048640 | 426 | 3 | 141 | 16.4 | 4.86 | Cha;Nua; Cya,b |
| GmGLYI-10 | Glyma.09 g004300 | Glyma.09 g004300.1 | 9 | 340275–344376 | 975 | 8 | 324 | 36.9 | 6.62 | Mta; Cyb |
| Glyma.09 g004300.2 | 891 | 9 | 296 | 33.5 | 6.13 | Cya,b | ||||
| Glyma.09 g004300.3 | 864 | 9 | 287 | 32.4 | 5.74 | Cya,b | ||||
| Glyma.09 g004300.4 | 840 | 8 | 279 | 31.5 | 6.97 | Cya,b | ||||
| Glyma.09 g004300.5 | 951 | 9 | 316 | 35.9 | 8.45 | Mta,b; Cyb | ||||
| GmGLYI-11 | Glyma.09 g193800 | Glyma.09 g193800.1 | 9 | 41830881–41834537 | 1041 | 9 | 346 | 39.0 | 6.68 | Cha,b,c; Mtb |
| Glyma.09 g193800.2 | 819 | 7 | 272 | 30.6 | 8.76 | Cha,c; Mtb | ||||
| GmGLYI-12 | Glyma.09 g226500 | Glyma.09 g226500.1 | 9 | 45136253–45137166 | 333 | 2 | 110 | 12.8 | 5.23 | Cyb |
| GmGLYI-13 | Glyma.11 g075000 | Glyma.11 g075000.1 | 11 | 5598647–5600229 | 579 | 3 | 192 | 22.1 | 4.97 | Cya,b |
| GmGLYI-14 | Glyma.11 g194200 | Glyma.11 g194200.1 | 11 | 26776807–26779603 | 747 | 8 | 248 | 28.5 | 5.48 | Poa; Cyb; Ecb |
| GmGLYI-15 | Glyma.11 g194300 | Glyma.11 g194300.1 | 11 | 26780838–26784795 | 702 | 8 | 233 | 26.5 | 9.16 | Cha,c; Mtb |
| GmGLYI-16 | Glyma.12 g079700 | Glyma.12 g079700.1 | 12 | 6241940–6246776 | 708 | 7 | 235 | 26.8 | 9.28 | Mta,b |
| Glyma.12 g079700.2 | 558 | 6 | 185 | 21.0 | 5.41 | Cya,b | ||||
| Glyma.12 g079700.3 | 525 | 8 | 174 | 20.0 | 9.69 | Mta,b | ||||
| GmGLYI-17 | Glyma.12 g167400 | Glyma.12 g167400.1 | 12 | 32196920–32200101 | 555 | 2 | 184 | 21.0 | 5.14 | Cya,b; Ecb |
| GmGLYI-18 | Glyma.13 g106600 | Glyma.13 g106600.1 | 13 | 22081599–22084230 | 630 | 5 | 209 | 23.4 | 6.49 | Cha,c; Ecb, Mtb |
| GmGLYI-19 | Glyma.13 g168200 | Glyma.13 g168200.1 | 13 | 28259866–28261852 | 504 | 3 | 167 | 19.0 | 5.46 | Eca; Cyb; Nub |
| GmGLYI-20 | Glyma.15 g009500 | Glyma.15 g009500.1 | 15 | 737953–739806 | 522 | 3 | 173 | 19.4 | 5.58 | Cha,b; Cyb |
| GmGLYI-21 | Glyma.15 g108400 | Glyma.15 g108400.1 | 15 | 8534420–8539477 | 864 | 8 | 287 | 32.4 | 5.74 | Cya,b |
| GmGLYI-22 | Glyma.16 g003500 | Glyma.16 g003500.1 | 16 | 194687–196274 | 549 | 2 | 182 | 20.6 | 5.22 | Cya,b; Chb |
| GmGLYI-23 | Glyma.17 g052700 | Glyma.17 g052700.1 | 17 | 4011185–4013445 | 621 | 5 | 206 | 22.9 | 7.08 | Cha,c; Mtb |
| GmGLYI-24 | Glyma.17 g115900 | Glyma.17 g115900.1 | 17 | 9163290–9164088 | 438 | 4 | 145 | 16.5 | 6.88 | Cha, Eca,b; Nub |
Abbreviations: CDS coding DNA sequence, Chro chromosome, PP polypeptide length, MW molecular weight, P I isoelectric point, bp base pair, aa amino acid, kDa kilodalton, Ch chloroplast, Cy cytosol, Ec extracellular, Mt mitochondria, Nu nucleus, Po peroxisome
aLocalization prediction by CELLO v.2.5 (http://cello.life.nctu.edu.tw/)
bLocalization prediction by pSORT (http://www.genscript.com/wolf-psort.html)
cChloroplast localization signal confirmed by ChloroP (http://www.cbs.dtu.dk/services/ChloroP/)