Table 5.
Sequence analyses of all putative GmGLYII proteins for the presence of conserved motifs and enzyme activity
| Sl. no | Proteins | Conserved metal binding motif (THHHXDH) | Active site motif (C/GHT) | Expected GLYII enzyme activity |
|---|---|---|---|---|
| 1 | GmGLYII-1.1 | Absent | Present | No |
| 2 | GmGLYII-2.1 | Present | Present | Yes |
| 3 | GmGLYII-2.2 | Present | Present | Yes |
| 4 | GmGLYII-2.3 | Present | Present | Yes |
| 5 | GmGLYII-2.4 | Absent | Present | No |
| 6 | GmGLYII-3.1 | Present | Present | Yes |
| 7 | GmGLYII-4.1 | Present | Present | Yes |
| 8 | GmGLYII-4.2 | Present | Present | Yes |
| 9 | GmGLYII-5.1 | Present | Present | Yes |
| 10 | GmGLYII-5.2 | Present | Present | Yes |
| 11 | GmGLYII-5.3 | Present | Present | Yes |
| 12 | GmGLYII-6.1 | Present | Present | Yes |
| 13 | GmGLYII-7.1 | Present | Present | Yes |
| 14 | GmGLYII-7.2 | Present | Present | Yes |
| 15 | GmGLYII-8.1 | Present | Present | Yes |
| 16 | GmGLYII-8.2 | Present | Present | Yes |
| 17 | GmGLYII-9.1 | Present | Present | Yes |
| 18 | GmGLYII-9.2 | Present | Present | Yes |
| 19 | GmGLYII-10.1 | Present | Present | Yes |
| 20 | GmGLYII-11.1 | Absent | Present | No |
| 21 | GmGLYII-12.1 | Present | Present | Yes |
| 22 | GmGLYII-12.2 | Present | Present | Yes |
| 23 | GmGLYII-12.3 | Present | Present | Yes |