Fig. 3.

A-homologous genes located on B chromosome. (a to d) Fluorescence in situ hybridization of B-located genes, pachytene stage chromosomes were probed with plasmids of B-located gene (red) and ZmBs (green). The signals of GRMZM2G013761B appeared on the DH2 heterochromatic region of B chromosome (a); the GRMZM2G054938B was located on the proximal euchromatic (PE) region near DH1 side (b); AF466202.2_FG007B had two foci on PE region (c); and GRMZM2G356653B was close to centromeric knob (d). The relative location of these four B chromosome genes were illustrated in (e). Arrowheads indicate the ZmBs signals, and arrows indicate the signals of B-located genes. Bar = 10 μm