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. 2016 Jan 2;9(1):e29384. doi: 10.5812/jjm.29384

Figure 11. A Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Analysis Performed to Detect Zinc Finger Nuclease (ZFN) Expression.

Figure 11.

Lanes 1 – 3, The Total Cell Protein (Tcp) of Escherichia coli top10 containing pZFN induced with 1 Mm Isopropyl Β-D-1-thiogalactopyranoside 4, 2, And 1 hour After Induction, Respectively; lane 4, the non-induced E. coli TOP10 containing pZFN; lane 5: the prestained protein ladder (Thermo Scientific, Waltham, MA, USA); lane 6, the TCP of the E. coli TOP10 containing pP15a, kanamycin-resistant ; lane 7 the TCP of E. coli TOP10 as negative controls. The ZFN protein band was not visible on SDS-PAGE due to the low levels of expression; B, thermo scientific prestained protein ladder size.