Figure 1.

Effects of ATP Treatment on the Differentiation of Mouse ESCs

(A and B) On day 14 of differentiation, cells were fixed and expression of the indicated proteins was examined. Scale bar indicates 25 μm.

(C) Cell extracts were prepared from cells on day 14 of differentiation. Twenty micrograms of protein was analyzed and expression of the indicated proteins was examined.

(D) The intensities of signals in (C) were assessed with ImageJ version 1.45n. The graph shows the mean of three independent experiments and the error bars indicate SE. The intensities of bands in control cells were set at 1. ^∗p < 0.05 (Student’s t test).

See also Figure S1.