Figure 1.

The Cell Cycle of ESCs Is Diverse

(A) Examples of lineage trees generated by live imaging of ESCs expressing Fucci vector. Each vertical line shows the fate of each cell plotted for every time point, where red and green indicate the G1 phase and the S/G2/M phase, respectively. Horizontal lines indicate cell division. Cells were sequentially numbered in the order they emerged (small black numbers). Timescale is on the left of the lineage tree. Green numbers indicate the cell-cycle length (hr).

(B) Histogram of the cell-cycle length of ESCs cultured in conventional media (n = 172 cell divisions in six lineages from two independent experiments). Black, the cells whose daughter cells divided within 30 hr; gray, the cells whose daughter cells died or did not divide within 30 hr.

(C) The cell-cycle lengths between mother and daughter, and between sisters were compared by the absolute value of the difference (n = 192 cell divisions in four lineages from two independent experiments). The difference between sisters was significantly small. m, mother; d, daughter. Bar graph represents the average with error bars of SE. Student’s t test was used for statistical analysis. ^∗p < 0.05.

(D) Quantification of the length of the G1 phase (red) and the S/G2/M phase (green) from the lineage trees as shown in (A) (n = 284 cell divisions in five lineages from two independent experiments).