Figure 6.

LPA Increases Precursor Proliferation In Vitro and In Vivo

(A) Histogram representing the number of neurospheres formed from primary adult dentate gyrus cells treated with 10 μM LPA or 15 mM KCl in the presence or absence of the LPA inhibitor DGPP. F₂₃ = 8.3, ^∗p < 0.05, ^∗∗p < 0.01, one-way ANOVA with Tukey's multiple comparison test, n = 4 experiments.

(B) Experimental design for in vivo LPA infusion and thymidine analog labeling.

(C–F) Histograms representing the number of surviving neurons (C) and the number of proliferating precursor cells (E) following 28 days of LPA infusion. Two-tailed Student's t test, ^∗p < 0.05, n = 11 animals (control group) and n = 12 animals (LPA group). Representative images of CldU (red) and NeuN (white) double-labeled cells (D) and IdU (red) single-labeled cells (F), DAPI-labeled nuclei (blue).

Data represent the mean ± SEM. Scale bars, 50 μm.