Figure 7.

LPA Signals via the AKT and MAPK Pathways In Vitro

(A) Experimental design for in vitro LPA signaling under proliferation conditions.

(B–D) There is a rapid phosphorylation of AKT (B) and MAPK (C) in response to LPA treatment of adherent neural precursor cells in vitro and primary dentate gyrus cells in vivo (D, n = 4 experiments).

(E) The activation of AKT signaling can be blocked by the specific LPA_1/3 antagonist DGPP.

(F) Experimental design for in vitro LPA signaling under proliferation conditions.

(G and H) There is a rapid phosphorylation of AKT and MAPK in response to LPA treatment in both differentiated adherent monolayers (G) and differentiated neurospheres (H, n = 3 experiments).