Table 2.
PCR type | Gene symbol | Primer sequences (5´-3´) |
---|---|---|
RT-PCR | β-ACTIN | F: CGTGACATTAAGGAGAAGCTGTGC |
R: CTCAGGAGGAGCAATGATCTTGAT | ||
hCAPZA3 | F: CAGTGGGTTTGGGCAGCTAC | |
R: CAGAATATTTTTGGCAGTGTTGG | ||
Quantitative Real-time PCR | CAPZA3 | F: CTGCCTGCTTTCATGGATAGAC |
R: TACTCTTTCCTTGTCCTTCCTG | ||
PLCζ | F: CAGATGCCTTGTTCAGTTATTGTC | |
R: GCCTTCATTTCCTACGGGTTG | ||
GAPDH | F: CCACTCCTCCACCTTTGACG | |
R: CCACCACCCTGTTGCTGTAG |