Table 3.

Bacterial strains and plasmids.

Strain/Plasmid	Relevant genotype and phenotype	Source/Reference
Strain
SM10λpir	thi recA thr leu tonA lacY supE RP4-2-Tc∷Mu λ∷pir	Laboratory strain
DH5αλpir	F− Δ(lacZYA–argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 λ∷pir	Hanahan (1983); Kolter et al. (1978)
AC-V66	C6709-1 El Tor biotype, lacZ∷ res–tet–res, SmR	This work
AC-V87	O395 classical biotype, lacZ∷ res1–tet–res1, SmR	This work
AC-V226	AC-V66 iviI∷pIVET5	This work
AC-V227	AC-V87 iviII∷pIVET5	This work
AC-V228	AC-V87 iviIII∷pIVET5	This work
AC-V229	AC-V66 iviIV∷pIVET5	This work
AC-V230	AC-V87 iviV∷pIVET5	This work
AC-V231	AC-V66 iviVI∷pIVET5	This work
AC-V232	AC-V66 iviVII∷pIVET5	This work
AC-V233	AC-V66 iviVIII∷pIVET5	This work
AC-V234	AC-V66 iviIX∷pIVET5	This work
AC-V235	AC-V66 iviX∷pIVET5	This work
AC-V236	AC-V66 iviXI∷pIVET5	This work
AC-V237	AC-V66 iviXII∷pIVET5	This work
AC-V238	AC-V87 iviXIII∷pIVET5	This work
AC-V167	O395 lacZ∷pGP704	This work
AC-V168	C6709-1 lacZ∷pGP704	This work
AC-V172	C6709-1 iviI∷pGP704	This work
AC-V176	O395 iviIII∷pGP704	This work
AC-V179	C6709-1 iviIV∷pGP704	This work
AC-V180	O395 iviV∷pGP704	This work
AC-V184	C6709-1 iviVI∷pGP704	This work
AC-V186	C6709-1 iviVII∷pGP704	This work
AC-V187	C6709-1 orf1∷pGP704	This work
AC-V188	C6709-1 orf2∷pGP704	This work
AC-V189	C6709-1 orf3∷pGP704	This work
AC-V191	C6709-1 iviX∷pGP704	This work
AC-V193	C6709-1 iviXI∷pGP704	This work
AC-V195	C6709-1 iviXII∷pGP704	This work
AC-V196	O395 iviXIII∷pGP704	This work
Plasmid
pGP704	oriR6K mobRP4, ApR	Miller and Mekalanos (1988)
pAC20	pACYC184∷lacZ∷res–tet–res	This work
pAC21	pACYC184∷lacZ∷res1–tet–res1	This work
pIVET5	pSKCAT2tnpR∷lacZYA’ trpAt	This work